Proteinase K resistant cores of prions and amyloids

The Transcellular Spread of Cytosolic Amyloids, Prions, and Prionoids

Recent reports indicate that a growing number of intracellular proteins are not only prone to pathological aggregation but can also be released and "infect" neighboring cells. Therefore, many complex diseases may obey a simple model of propagation where the penetration of seeds into hosts determines spatial spread and disease progression. We term these proteins prionoids, as they appear to infe...

Isolation of Proteinase K-Sensitive Prions Using Pronase E and Phosphotungstic Acid

Disease-related prion protein, PrP(Sc), is classically distinguished from its normal cellular precursor, PrP(C), by its detergent insolubility and partial resistance to proteolysis. Molecular diagnosis of prion disease typically relies upon detection of protease-resistant fragments of PrP(Sc) using proteinase K, however it is now apparent that the majority of disease-related PrP and indeed prio...

Direct and selective elimination of specific prions and amyloids by 4,5-dianilinophthalimide and analogs.

Mechanisms to safely eliminate amyloids and preamyloid oligomers associated with many devastating diseases are urgently needed. Biophysical principles dictate that small molecules are unlikely to perturb large intermolecular protein-protein interfaces, let alone extraordinarily stable amyloid interfaces. Yet 4,5-dianilinophthalimide (DAPH-1) reverses Abeta42 amyloidogenesis and neurotoxicity, w...

Prions of yeast are genes made of protein: amyloids and enzymes.

Comparative Studies on the Interaction of Proteinase-K with Nano-CuO and Copper Ions

The interaction of copper oxide nanoparticles and copper ions and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics measurements. The aim of this study was to evaluate the effect of nanoparticles of CuO for proteinase K. In this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase K sta...